Figure 5.

The BoHV-1qmv Sub-RVFV vaccine virus-expressed Gn-GMCSF and Gc proteins form the Gn–Gc complex in the infected cells in vitro. KOP-R cells were infected with the BoHV-1qmv Sub-RVFV. Infected cell lysates were immunoprecipitated with (A) FLAG tag- or V5 tag-specific antibodies followed by immunoblotting with RVFV Gc- or Gn-specific rabbit polyclonal antibodies to identify the co-immunoprecipitated proteins. (B) Similarly, the RVFV Gn-immunoprecipitated proteins were immunoblotted with FLAG tag- or V5 tag-specific antibodies to recognize the co-immunoprecipitated proteins.