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. 1998 Sep;64(9):3480–3485. doi: 10.1128/aem.64.9.3480-3485.1998

FIG. 3.

FIG. 3

In situ identification of nitrifying bacteria in aggregate sections demonstrating their spatial distribution. Images are composed of overlays of phase-contrast microscopic images and two confocal microscopic images. (A) Simultaneous in situ hybridization with CY5-labeled probe NSV443 and CY3-labeled probe NSR1156. Cells of Nitrosospira spp. are shown in blue; cells of Nitrospira-like bacteria are red. (B) Simultaneous in situ hybridization with CY5-labeled probe NSV443 and CY3-labeled probe NSR447. Cells of Nitrosospira spp. are blue; a smaller subpopulation of Nitrospira spp. is visualized in red. (C) Simultaneous in situ hybridization with CY5-labeled probe NSV443 and CY3-labeled probe NSR1156 displaying the morphology and close association of ammonia- and nitrite-oxidizing bacteria.