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Production of AHLs by biofilms of P. aeruginosa NSM35 growing on all-silicone catheters. Positive reactions for AHLs are visible in colonized catheter sections (A) and in sections that had been cleaned of biofilm by sonication and then autoclaved (C). No evidence of AHL activity is visible in sections of fresh unused catheters (B), catheter sections that had been exposed to urine and then sonicated and autoclaved (E), autoclaved biofilm extracts (D), or samples of urine (F).
