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Agarose gel with PCR products from 10-fold serial dilutions of ruminal particles from sheep T1 (trial I). Lanes and fold dilutions of ruminal samples are as follows: A, 10−1; B, 10−2; C, 10−3; D, 10−4; E, 10−5. Aliquots of 1 μl were used in each reaction. Lane F is the positive control product, and lane G contains λ HindIII size markers. The diffuse lower band was a commonly observed artifact in PCR that used complex biological mixtures as a source of template.
