Table 2.
BAS Group | Spectrophotometric Method | Assay, % | |
---|---|---|---|
PE | PE + Arg | ||
Hydroxycinnamic acids | In terms of chlorogenic acid (λ = 327 nm) | 11.54 ± 0.11 | 7.10 ± 0.07 |
In terms of chlorogenic acid (λ = 525 nm); chromogenic reagent: sodium nitrite and sodium molybdate | 13.59 ± 0.63 | 8.10 ± 0.37 | |
Flavonoids | In terms of rutin (λ = 417 nm) | 4.01 ± 0.26 | 2.53 ± 0.14 |
In terms of hyperoside (λ = 425 nm) |
4.94 ± 0.46 | 3.19 ± 0.45 | |
Total polyphenols | In terms of gallic acid (λ = 270 nm) | 17.16 ± 0.29 | 4.94 ± 0.30 |
Chromogenic reagent: Folin & Ciocalteu′s Phenol Reagent (λ = 765 nm) | 19.18 ± 0.43 | 7.59 ± 0.56 | |
Amino acids | In terms of leucine (λ = 573 nm); chromogenic reagent: ninhydrin solution | 0.88 ± 0.09 | 5.60 ± 0.38 |