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. 2023 Nov 9;12(22):3815. doi: 10.3390/plants12223815

Figure 3.

Figure 3

Validation of the inhibition of miR2118b to candidate targets via transient expression in rice protoplasts. (A) Diagram of the construction of LUC-42700 and LUC-42700m3; The red CTA in box was inserted into the binding sites between 10-11 bases to interrupt the specific binding between miRNA and target mRNA. (B) fluorescence intensity after the co-transformation of pUC-MIR2118 with different LUC fusion vectors in the rice protoplast system; (C) quantitative analysis of LUC activity in (B). Error bars represent the standard deviations. Asterisks denote a significant difference between co-transformation with LUC-fusion and with LUC-fusion mutation (Student’s t-test, ** p < 0.01). This experiment was repeated at least three times with similar results.