Table 3.
Primer sequences in this work.
| Primer | Sequence (5′-3′) | Restriction Enzyme | Purpose |
|---|---|---|---|
| MIR2118-F | GGATCCTATACGC ATGGTGTTTCACTTAC |
BamH I, Pst I | Amplified MIR2118 gene |
| MIR2118-R | CTGCAGTTTAGAGGG ACAGAAGAGTTTGA |
||
| 42700-F | tcgacGGGGAATGGGTG GCATTGGGAActgca |
Pst I, Sal I | Construction of LUC-42700 fusion vector |
| 42700-R | gTTCCCAATGCCACCC ATTCCCCg |
||
| 42700m3-F | tcgacGGGGAATGGGTG ctaGCATTGGGAActgca |
Pst I, Sal I | Construction of LUC-42700m3 fusion vector |
| 42700m3-R | gTTCCCAATGCtagCACCCATTCCCCg | ||
| 05600-F | tcgacAGGGGATGGGAGGCATCGGTAActgca | Pst I, Sal I | Construction of LUC-05600 fusion vector |
| 05600-R | gTTACCGATGCCTCCCA TCCCCTg |
||
| 05600m3-F | tcgacAGGGGATGGGAG ctaGCATCGGTAActgca |
Pst I, Sal I | Construction of LUC-05600m3 fusion vector |
| 05600m3-R | gTTACCGATGC tagCTCCCATCCCCTg |
||
| 37290-F | tcgacGGGGAAUGGGU GGUAUUGGGAActgca |
Pst I, Sal I | Construction of LUC-37290 fusion vector |
| 37290-R | gTTCCCAATACCACC CATTCCCCg |
||
| 37290m3-F | tcgacGGGGAAUGGGUG ctaGUAUUGGGAActgca |
Pst I, Sal I | Construction of LUC-37290m3 fusion vector |
| 37290m3-R | gTTCCCAATA tagCCACCCATTCCCCg |