Figure 1.
Formation of stable nanodisc using cNW50 incorporating CoPoP as a His-tag protein-binding lipid, and QS-21 and MPLA as immune stimulants. (A) Size exclusion chromatogram of empty nanodiscs containing MPLA, QS-21, and CoPoP, with a schematic insert of the proposed structure of the formed nanodisc. (B) Negatively stained transmission electron micrograph of formed nanodiscs, scale bar = 100 nm. (C) Stability of formed nanodisc at 4 °C for 28 days as tested by size exclusion chromatography.