Table 1.

Human primer sequences used for Real-Time PCR analysis of human skeletal muscle.

Gene	Accession Number	Sequence
ACC-α	NM_198836.2	Forward (5′–3′) CTGGAGCCCTCAACAAAGTC Reverse (5′–3′) CCAGTGCAGGACAGTGAAAA
AMPK-α	XM_016919269	Forward (5′–3′) AACTGCAGAGAGCCATTCACTTT Reverse (5′–3′) GGTGAAACTGAAGACAATGTGCTT
Cyclophilin	XM_024747056	Forward (5′–3′) CATCTGCACTGCCAAGACTGA Reverse (5′–3′) TTCATGCCTTCTTTCACTTTGC
FoxO1	XM_522749	Forward (5′–3′) TCATGGATGGAGATACATTGGATT Reverse (5′–3′) TCCTGCTGTCAGACAATCTGAAG
GAPDH	XM_024733357	Forward (5′–3′) CAA CGA CCA CTT TGT CAA GC Reverse (5′–3′) TTA CTC CTT GGA GGC CAT GT
IRS-1	NM_000208	Forward (5′–3′) GTTTCCAGAAGCAGCCAGAG Reverse (5′–3′) TGAAATGGATGCATCGTACC
IRS-2	NM_003749.2	Forward (5′–3′) ACGCCAGCATTGACTTCTTGT Reverse (5′–3′) TGACATGTGACATCCTGGTGATAA
PGC1-α	NM_013261.4	Forward (5′–3′) CAAGCCAAACCAACAACTTTATCTCT Reverse (5′–3′) CACACTTAAGGTGCGTTCAATAGTC
PI3K	NM_181504.3	Forward (5′–3′) GGAAGCAGCAACCGAAACAA Reverse (5′–3′) TTCGCCGTCCACCACTACA
PPAR-α	XM_024452253	Forward (5′–3′) GAAGCTGTCCTGGCTCAGAT Reverse (5′–3′) GGGGACCACAGGATAAGTCA

ACC-α, acetyl-CoA carboxylase alpha; AMPK-α, 5′ AMP-activated protein kinase alpha; FoxO1, forkhead box protein O1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IRS-1, insulin receptor substrate 1; IRS-2, insulin receptor substrate 2; PGC1-α, peroxisome-proliferator-activated receptor-gamma coactivator 1-alpha; PI3K, phosphoinositide 3-kinase; PPAR-α, peroxisome-proliferator-activated receptor alpha.