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. 2023 Oct 29;10(11):636. doi: 10.3390/vetsci10110636

Table 1.

List of primers used for the amplification of rickettsial fragments in various ticks collected from camels, goats, and sheep in the present study.

Gene Primer Sequence Amplicon Size PCR Condition Study
gltA CS-78 GCAAGTATCGGTGAGGATGTAAT 401 bp 95 °C 3 min, 40× (95 °C 15 s, 48 °C 30 s, 72°C 30 s), 72 °C 7 min [54]
CS-323 GCTTCCTTAAAATTCAATAAATCAGGAT
ompA Rrl9O.70 ATGGCGAATATTTCTCCAAAA 532 bp 95 °C 3 min, 35× (95 °C 20 s, 48 °C 30 s, 63 °C 2 min), 72 °C 7 min [55]
Rrl9O.602 AGTGCAGCATTCGCTCCCCCT
ompB 120-M59 CCGCAGGGTTGGTAACTGC 862 bp 95 °C 3 min, 40× (95 °C 30 s, 50 °C 30 s, 68 °C 90 s), 68 °C 7 min [56]
120-807 CCTTTTAGATTACCGCCTAA