Figure 1.

Extraction and characterization of lipopolysaccharides from Brucella suis vaccine strain 2 (S2). (A) Brucella suis vaccine strain 2 (Brucella suis S2) was isolated by TSA and amplified by TSB. The structure distribution of Brucella suis S2 LPS (1 × 10⁹ CFU/mL) extracted with a bacterial lipopolysaccharide extraction kit was detected with the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) silver staining method using 1 mg/mL of commercial E. coli LPS as a control. M: Marker, E-LPS: E. coli lipopolysaccharide, B-LPS: Brucella suis S2 lipopolysaccharide, 1 refers to the O-antigen, 2 and 3 refer to the lipid A core. (B) Lipopolysaccharide activity of Brucella suis S2 was detected with a Limulus amebocyte lysate kit. The data represent the mean ± standard deviations from three independent experiments.