Skip to main content
. 2023 Nov 13;15(11):2251. doi: 10.3390/v15112251

Figure 1.

Figure 1

Schematics of GeCKO-A549 cells generation, selection, NGS, and data analysis. A549 cells were transduced with the lentivirus-CRISPR-Cas9 library to generate GeCKO-A549 cells. Then, the GeCKO-A549 cells were subjected to three rounds of infection with the RVFV MP-12 (1 MOI) virus. The genomic DNA of round 0 GeCKO-A549 cells, round 1, and round 3 GeCKO-A549cells were sequenced using the Illumina NextSeq 550 platform. The output NGS data were analyzed using the MaGeCK program to generate the list of genes involved in RVFV replication.