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. 2023 Nov 13;15(11):2251. doi: 10.3390/v15112251

Figure 2.

Figure 2

Validation of gene hits via siRNA gene knockdown study. A549 cells were transfected with 50 nM of siRNAs. At 48 h post-transfection, the cells were infected with RVFV MP-12 virus at 0.1 MOI. At 24 h post-infection, the supernatant was collected and titered using plaque assay. NTC-non-target control siRNA; si46N-anti-RVFV siRNA; and WDR7, SLC35B2, EXOC4, LRP1, EMC3, CT47A1 gene-specific siRNAs were transfected. Each bar represents the average virus titer (pfu/mL) along with the corresponding standard deviation. Statistical analysis was performed on two independent experiments with four replicates for each, using the Mann–Whitney U test and independent Student’s t-test (** p-value < 0.005, *** p-value < 0.001).