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. 2023 Nov 13;15(11):2251. doi: 10.3390/v15112251

Figure 4.

Figure 4

Viral RNA accumulation at various time points post-infection in WDR7 knockout (KO) cells. CT and WDR7 KO 1 cells were infected with (A) RVFV MP-12 vaccine strain or (B) LACV, both at 0.1 MOI. Total cellular RNA was harvested at various hours post-infection (h pi). One-step RT-qPCR was performed to detect the level of viral RNA using the PGK1 gene as an internal control. CT and WDR7 KO 1 cells were utilized. Each bar graph represents the average fold change in viral RNA expression, along with the corresponding standard deviation. Statistical analysis was performed on three independent experiments with two to three technical replicates for each, using the Mann–Whitney U test and independent Student’s t-test (* p-value < 0.05, *** p-value < 0.001, ns, non-significant).