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HLA-E expression in tumor cells after treatment with IFN-γ in vitro. IFN-γ-induced HLA-E upregulation was demonstrated by quantitative real-time PCR (A), western blot (B), and flow cytometry(C). IFN-γ: the cells were treated with 25ng/mL recombinant human IFN-γ for 24 h; Control: the cells were cultured without IFN-γ adding. Open histogram: anti-HLA-E antibody; filled histogram: isotype control. ***P < 0.001. Data shown represented one of three independent experiments
