Fig. 1.

Effect of hypoxia on the expression of a HIF-1α, HIF-2α and b several HIF-target genes in H2030-BrM3 cells. a Immunocytology for HIF-1α and HIF-2α evaluated after 3 h or 24 h in hypoxic or normoxic condition. b Gene expression of HIF-target genes evaluated by real-time RT-PCR (TUBB3, VEGF-A, SLC2A1, CCDN1, SERPINE1 and CA-IX) in H2030-BrM3 cells submitted or not to hypoxia (1% O2) during 40 h. Median ± IQR (Interquartile Range), n = 4 independent experiments, * p < 0.05, one sample t-test vs theorical value of 1