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. 2023 Nov 25;14:7733. doi: 10.1038/s41467-023-43513-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Breeding strategy to generate embryonic kidneys lacking Acly expression in the CM cells (a). H&E-stained control kidney (b), heterozygous kidneys (c), and mutant kidneys (d). The nephrogenic zone is marked with a dashed line at 20X. Detailed structures can be observed at 40X magnification. The nephrogenic zone is narrower in heterozygous and mutant kidneys. Detailed structures observed at 40X magnification revealed dilations at UB ampullae (black arrow) and the presence of some nascent nephrons (red arrows), CM around dilated UB ampullae appears depleted and almost absent in some areas of mutant embryos. Also, notice expansion of interstitial tissue (red asterisk) (d). Glomeruli counts at P0 in control (blue, n = 11 independent kidneys), heterozygous kidneys (gold, n = 7 independent kidneys), and mutant embryos (grey, n = 7 independent kidneys) (e), scale bar 100 µm. A Kruskal-Wallis test for multiple comparisons was used to compare groups and p-values are shown. Source data are provided as a Source Data file.