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. 2023 Nov 25;14:7733. doi: 10.1038/s41467-023-43513-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — Visualization of nephrogenic niches of control kidneys (a, d), heterozygous (b, e), and mutant kidneys (c, f) at P0. E16.5 kidneys are displayed as control kidneys (g), heterozygous (h), and mutant kidneys (i). E14.5 kidneys are shown as control kidneys (j), heterozygous (k), and mutant kidneys (l) Cyan arrows point to dilated UB ampullae; white arrows show areas with depleted cap-mesenchyme; yellow arrows show expansion of Wnt4 towards the cap-mesenchyme. Five embryonic kidneys per genotype per stage were used in this experiment. Embryos from at least two distinct litters per stage and genotype were sectioned and stained. Scale bar 100 µm.