Table 3. L-asparaginase activity of expressed enzyme in soluble fraction of transformed E. coli .

Sample	Contents	L-asparaginase activity (IU ± SD)	L-glutaminase activity (IU ± SD)
A	Soluble fraction of cell lysate prepared from transformed E.coli BL21 pLysS	25.8 ± 2.5	2.7 ± 1.1
B	Soluble fraction of cell lysate prepared from transformed E.coli Origami	95.4 ± 7.5	13.3 ± 1.0
C	Commercial enzyme 0.5 mg/mL (positive control)	83.9 ± 11.0	53.3 ± 1.9

L-asparaginase activity (IU) values are for 0.1 mL of crude soluble fraction of cell lysate or 0.1 mL of pure commercial enzyme prepared at 0.5 mg/mL concentration.