Fig. 2.
ATF6 upregulates CBS expression. A The mRNA levels of CBS and CTH were analyzed by qPCR in liver tissues from WT and ATF6−/− mice fed with HFD. B The expression of CBS and CTH were evaluated by western bolt in liver tissues from WT and ATF6−/− mice. C The mRNA levels of CBS were analyzed by qPCR in HepG2 cells with ATF6 overexpression and control ones, respectively. D The expression of CBS was evaluated by western blot in HepG2 cells with ATF6 overexpression and control ones. The asterisks (***) marked in the graph represents the significant differences (p*** < 0.001) in two groups. (n = 5–6). WT wildtype, NC negative control