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. 2023 Nov 25;23:293. doi: 10.1186/s12935-023-03129-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Exam the downstream targets of ALKBH5 in Gastric Cancer. (A) Extraction of MeRIP-seq data comparing the distribution of genes with differential expression and degree of m6a modification in AGS cells with those in the control group. (B) HOMER highest shared motifs with m 6 A-seq peaks in AGS cells with or without ALKBH5 knockdown. (C) In the RNA-seq dataset (m 6 A-seq input library) we selected 265 genes that showed more than 8-fold numerical differences. In shALKBH5 there are 5,180 unique m 6 A peaks for 1591 genes. The two were intersected and 45 genes were identified. (D) RT-qPCR was used to detect the expression of the 16 genes screened out in ALKBH5 knockdown versus control cells (Data are expressed as mean ± SD. * p < 0.05), and assays were repeated 3 times biologically. (E) MeRIP-seq data comparing the expression of the m 6 A-seq peak at the CHAC1 locus in ALKBH5 knockdown and control cells