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. 2023 Dec 1;14(6):2177–2192. doi: 10.14336/AD.2023.0503

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Figure 6. — eCAFs significantly promoted macrophage chemotaxis by activating the Akt signalling pathway in macrophages. (A) The effect of POSTN overexpression in CAFs on Akt phosphorylation in macrophages was detected by Western blotting. A: OE-POSTN-CAFs: macrophages treated with conditioned medium from CAFs overexpressing POSTN; B: OE-NC-CAFs: macrophages treated with conditioned medium from control CAFs. (C-D) The effect of POSTN-silenced CAFs on Akt phosphorylation in macrophages was detected by Western blotting. C: si-POSTN-CAFs: macrophages treated with POSTN-silenced CAF-conditioned medium; D: si-NC-CAFs: macrophages treated with control CAF-conditioned medium. (E) Effect of Akt phosphorylation inhibitor on chemotactic macrophages of CAFs (n = 4). Left top panel: Treatment of macrophages with control CAF conditioned medium with Akt phosphorylation inhibitor or DMSO; left bottom panel: Treatment of macrophages with overexpressing POSTN CAF conditioned medium with Akt phosphorylation inhibitor or DMSO; F: Statistical data. ***, p<0.001; ns, no significant difference; T test).