TABLE 2.
Primer | Sequencea | Reference(s) |
---|---|---|
Forward | 5′-TG YGA YCC NAA RGC NGA-3′ | 44, 45 |
Reverse | 5′-AD NGC CAT CAT YTC NCC-3′ | 44, 45 |
NHA1 | 5′-TCC ACT CGT CTG ATC CTG-3′ | This study |
NHA2 | 5′-CTC GCG GAT TGG CAT TGCG-3′ | This study |
GC clampb | 5′-CGCCCGCCGCGCCCCGCGCCCGT CCCGCCGCCCCCGCCCG-3′ | 19 |
Y = T or C; N = A, C, G, or T; R = A or G; and D = A, G, or T.
The GC clamp was attached to the 5′ end of the primer NHA1.