FIG. 3.

Southern hybridization of undigested and HindIII-digested plasmid DNAs from transductants, E. coli RM1259, donor strain HOPE-1, and recipient strain HSIC, probed with the nptII probe. Lanes 1 through 20, plasmid DNAs from transductants; lanes 21 and 22, plasmid DNA from E. coli RM1259; lanes 23 and 24, plasmid DNA from donor strain HOPE-1; lanes 25 and 26, plasmid DNA from recipient strain HSIC. Odd-numbered lanes contained undigested plasmid DNA, whereas even-numbered lanes contained HindIII-digested DNA. Molecular weights (M) (in kilobases) are indicated on the left.