FIG. 6.

Autoradiograph after Southern transfer of an agarose gel containing PCR products obtained with primers JP44 and JP52. The template DNAs were plasmid DNAs of transductants (lanes 1 through 7), plasmid DNAs of indigenous antibiotic-resistant bacteria (lanes 8 through 12), and pQSR50 DNA (lane 13). Lane 14 was a negative control (no template DNA). The preparations were hybridized with the nptII probe. Lane M contained a molecular weight marker; sizes (in base pairs) are indicated on the left.