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PCR amplification performed with primers JP64 and JP65 and EcoRI-digested amplification products. The template DNAs were DNAs from plasmids of transductants (lanes 1 through 10), indigenous antibiotic-resistant bacteria (lanes 11 and 12), and pQSR50 (lanes 13 and 14). Odd-numbered lanes contained uncut DNA, and even-numbered lanes contained EcoRI-cut DNA. Lane M contained a molecular weight ladder; sizes (in base pairs) are indicated on the right.
