TABLE 3.
Marine phage transduction with bacterial isolates as recipients
Donor | Transducing phage(s) | Recipient | MOI | Frequencies of transduction
|
|
---|---|---|---|---|---|
No. of transductants/PFU | No. of transductants/CFU | ||||
HOPE-1 | UV-treated T-φHSIC lysate | HSIC (10 ml) | 0.01 | 5.13 × 10−9 | 4.02 × 10−10 |
HSIC (100 ml) | <0.005 | <2.6 × 10−11 | |||
0.01 | 1.33 × 10−7 | 6.8 × 10−10 | |||
0.05 | 1.33 × 10−8 | 6.8 × 10−10 | |||
0.5 | <2.6 × 10−11 | ||||
Untreated T-φHSIC lysate | HSIC (10 ml) | 0.01–10 | NDa | ||
HOPE-2 | UV-treated and untreated T-φD1B lysates | D1B (10 ml) | 0.01–5 | ND |
ND, not detected.