TABLE 2.
Prepn | Sp act (U/mg) with:
|
|||||
---|---|---|---|---|---|---|
Azo dyes
|
Phthalocyanine dyes
|
|||||
RV5 | RB5 | RO96 | RR198 | RB38 | RB15 | |
B. adusta MnP1 | 6.6 | 6.1 | 5.8 | 3.2 | 7.2 | 9.7 |
P. eryngii MnPL1 | 8.2 | 5.4 | 6.8 | 3.7 | 9.3 | 10.8 |
P. eryngii MnPL2 | 8.1 | 5.4 | 8.1 | 3.2 | 10.0 | 10.9 |
P. chrysosporium MnP1 | 0.09 | Nb | N | N | 0.22 | 0.12 |
P. chrysosporium MnP1 + 0.3 mM MnSO4 | 0.46 | N | N | N | N | N |
B. adusta LiP2 | 0.2 | 0.3 | 0.13 | 0.09 | 0.67 | 1.1 |
B. adusta LiP2 + 2 mM veratryl alcohol | 5.6 | 5.1 | 3.9 | 9.6 | 5.3 | 9.5 |
The reaction mixtures contained 0.1 mM H2O2 and 0.1 M tartrate at pH 3.5 (azo dyes) or pH 3.75 (phthalocyanines). For the MnP reactions dye concentrations of 30 μM (azo dyes) and 20 μM (phthalocyanines) were used. In the case of LiP reactions a lower dye concentration (10 μM) was used to avoid inhibition by high substrate concentrations.
N, negligible (<10−3 U/mg).