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. 1998 Aug;64(8):2794–2799. doi: 10.1128/aem.64.8.2794-2799.1998

FIG. 1.

FIG. 1

Schematic diagram of the construction of cutinase variant gene (cutiX) integration vectors. pcutiX is a model expression vector, in which cutiX is regulated by the exlA (endoxylanase II) promoter (P) and terminator (T). pUR7981 is a derivative of pJB8 containing the pyrG gene with a defined SalI mutation, pyrG*. For random integration, more than four cutiX expression cassettes were cloned into the HindIII site of pUR7981, resulting in pUR7981-HX. Targeted integration at the pyrG locus was performed with pUR7981-SX, with pUR7981 containing two cutiX expression cassettes at the SalI site.