TABLE 2.
Expression and production of cutinase variants by A. awamori
| Strain | Mutation(s) | Cutinase gene copy no. | Level of cutinase mRNAa | Cutinase concn (mg/liter)b |
|---|---|---|---|---|
| AW85 | 1 | 1 | 14 ± 3 | |
| AW91 | 2 | 2–5 | 74 ± 19 | |
| AW23 | L51S | 2 | 2–5 | 6 ± 2 |
| AW91-16 | 10–15 | 5–10 | 55 ± 3 | |
| AW23-16 | L51S | 2–5 | 2–5 | 1 ± 1 |
| AW20 | E201K | 5–10 | 5–10 | 51 ± 6 |
| AW28 | G82A, A85F, V184I, A185L, L189F | 5–10 | 2–5 | 66 ± 10 |
| AW34 | A29−, S30− | 2–5 | 2–5 | 62 ± 18 |
| AW48-15 | T179Y | 5–10 | 2–5 | 25 ± 11 |
| AW48-04 | T179Y | 15–30 | 2–5 | 6 ± 2 |
| AW55 | W69Y | 10–15 | 5–10 | 50 ± 14 |
a
mRNA level relative to the mRNA level in single-copy strain AW85, which was defined as 1.
b
Mean ± standard deviation from two or more independent shake flask induction experiments. Enzyme levels were obtained by relating the PNPB activities of samples to the activity of a purified wild-type cutinase sample having a known concentration.