TABLE 5.
Results of the first screening experiment performed with log-phase cultures (20-h cultures in 0.1× ZoBell broth [see text])
| Test organism | Strain Ya
|
P. carrageenovorab
|
Control activee | ||
|---|---|---|---|---|---|
| MSE (time)c | Recoveryd | MSE (time)c | Recoveryd | ||
| Gymnodinium catenatum | Lysis (1 h 55 min) | No | Rounded (3 h 50 min) | No | Yes |
| Gymnodinium sanguineum | Lysis (1 h 30 min) | No | Disintegrated (15 min) | No | No |
| Gyrodinium sp. strain 1 | Lysis (3 h 50 min) | No | Rounded (1 h 30 min) | Yes | Yes |
| Gyrodinium cf. uncatenum | Lysis (1 h 55 min) | No | Rounded (3 h 50 min) | Yes | Yes |
| Gyrodinium sp. strain 2 | Lysis (1 h 55 min) | No | Disintegrated (5 h 10 min) | No | No |
| Gyrodinium sp. strain 3 | Lysis (1 h 30 min) | No | Rounded (30 min) | No | Yes |
| Alexandrium minutum | Ecdysis (50 min) | Yes | Rounded (50 min) | Yes | Yes |
| Alexandrium catenella | Ecdysis (50 min) | Yes | Rounded (1 h 30 min) | Yes | Yes |
a
The initial strain Y concentration was 0.91 × 107 cells per ml.
b
The initial P. carrageenovora concentration was 2.11 × 107 cells per ml.
c
Lysis, rapid leaking and cell contents destroyed; disintegrated, cell membrane disrupted and internal contents in disarray; rounded, flagella not visible and each cell resembled a temporary resting stage; ecdysis, theca shed (thecate species). The times are the times when the MSE were first observed.
d
Swimming cells were present in wells after 2 days.
e
The control was considered to have had no effect if the test organism was still active after 2 days.