TABLE 1.
Bacterial strains and plasmids used
| Strain or plasmid | Characteristics | Reference or source |
|---|---|---|
| E. coli strains | ||
| C2110 | polAI | 27 |
| HB101 | recA13 | 6 |
| DH5α | recA1 | 4 |
| Marine strains | ||
| Achromobacter sp. | Georgia salt marsh isolate, Rifr | 52a |
| Pseudomonas nautica ATCC 35188 | Rifr | ATCCa |
| Pseudomonas duodoroffii ATCC 27123 | Rifr | ATCC |
| Roseobacter sp. strain 164 | San Diego salt marsh isolate, source of repSD164 | This study |
| Serratia rubidaea ATCC 27164 | Rifr | ATCC |
| Vibrio sp. | Georgia salt marsh isolate, Rifr | 52 |
| Vibrio sp. strain 41 | San Diego salt marsh isolate, source of repSD41 | This study |
| Vibrio sp. strain 121 | San Diego salt marsh isolate, source of repSD121 | This study |
| Vibrio sp. strain 172 | San Diego salt marsh isolate, source of repSD172 | This study |
| Plasmids | ||
| pUC4K | Source of Tn903 npt gene | 59 |
| pRK2013 | ColE1 replicon, RK2 Tra+ | 16 |
| pFF1 | Mini-RK2 replicon, bla cat oriT | 17 |
| pBR325 | pBR322 + Cmr (ColE1) | 44 |
| pTM41 | pBR325 + repSD41 | This study |
| pTM121 | pBR325 + repSD121 | This study |
| pTM164 | pBR325 + repSD164 | This study |
| pTM172 | pBR325 + repSD172 | This study |
a
ATCC, American Type Culture Collection.