TABLE 5.

Influence of Trypticase on incorporation of ¹⁵NH₃ by S. bovis ES1^a

Parameter	Value for growth medium						SEDc
	Basal	Basal + Trypticase (1 g/liter)	Basal + Trypticase (5 g/liter)	Basal + Trypticase (10 g/liter)	Basal + Trypticase (30 g/liter)	Complete M2
NH3 concn (g of N/liter)
Initial	0.156	0.154	0.190	0.238	0.376	0.353	0.005
Final	0.114	0.125	0.188	0.224	0.343	0.337	0.008
Enrichment in NH3 (atom%)
Initial	34.2	34.3	33.5	31.1	25.9	26.8	0.56
Final	30.7	28.9	28.9	28.6	23.3	25.9	1.78
Microbial N formed (g/liter)	0.058	0.091	0.097	0.123	0.153	0.088	0.007
Enrichment in microbial N (atom%)	38.5	20.1	9.3	7.0	3.9	3.2	0.80
Proportion of microbial N derived from ammonia	1.19	0.64	0.30	0.23	0.16	0.12	0.022
Proportion of microbial amino acids derived from ammoniab
Ala	1.08	0.64	0.12	0.08	0.03	0
Gly	1.09	0.56	0.04	0.01	0	0
Val	0.99	0.30	0	0	0	0
Leu	1.00	0.40	0.06	0.04	0.02	0.01
Ile	1.00	0.33	0.02	0	0	0
Pro	0.99	0.05	0	0	0	0
Ser	1.07	0.47	0.01	0	0.01	0
Thr	1.08	0.66	0.07	0	0	0
Phe	1.01	0.15	0.05	0.04	0.03	0
Asp	1.09	0.66	0.25	0.17	0.11	0.05
Glu	1.09	0.80	0.42	0.30	0.16	0.08
Tyr	1.02	0.47	0.08	0.04	0.02	0
Mean proportion of amino acid N derived from ammonia	1.04	0.46	0.09	0.06	0.03	0.01	0.022

^aResults are mean values from three separate cultures. 

^bStandard errors of difference for proportion of individual microbial amino acids derived from ammonia are as follows: treatment, 0.007; amino acid, 0.010; treatment × amino acid, 0.238 (degrees of freedom, 142). 

^cSED, standard errors of differences of means.