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Influence of hydroxylamine concentration on hydrolysis of both benzamide and benzohydroxamic acid. The initial benzamide concentration and the amidase concentration in the reaction media were 5 mM and 44 μg/ml, respectively. The following four initial hydroxylamine concentrations were tested: 0 mM (A), 10 mM (B), 50 mM (C), and 500 mM (D). Symbols: ○, amount of benzoate formed; •, amount of benzohydroxamic acid.
