TABLE 4.
Kinetic constants for amidase-catalyzed acyl transfer reactions with l-enantiomers, d-enantiomers, and racemic mixtures of several α-substituted amides
| Compound | Structural formula | kcat (s−1) | Kmamide (mM) | KmNH2OH (mM) | pH |
|---|---|---|---|---|---|
| l-Methioninamide |  |
245 | 5.4 | 36 | 8 |
| dl-Methioninamide | 180 | 6.4 | 75 | 8 | |
| l-Leucinamide | 63 | 1.3 | 39 | 8 | |
| dl-Leucinamide | 24 | 2.4 | 187 | 8 | |
| l-Phenylalaninamide | 8.1 | 0.8 | 19 | 8 | |
| dl-Phenylalaninamide | 6.2 | 1.1 | 19 | 8 | |
| l-Lactamide | 67 | 33 | 41 | 7 | |
| dl-Lactamide | 426 | 51 | 507 | 7 | |
| d-Lactamide | 2,504 | 270 | 2,923 | 7 | |
| l-Alaninamide | 66 | 12 | 25 | 8 | |
| d-Alaninamide | 3,353 | 379 | 1,939 | 8 |