TABLE 5.

Acyl transfer activities and spontaneous chemical reactions obtained with different amides, carboxylic acids, and methyl esters as acyl donors^a

Acyl donor	Acyl transfer activity		Chemical reaction
	Optimum working pH	Sp act (U/mg)	pH	Activity (μmol/min/ ml)
l-Alaninamide (100 mM)	8	60		0
l-Alanine (100 mM)		0		0
l-Methioninamide (100 mM)	8	160		0
l-Methionine (100 mM)		0		0
l-Methionine methyl ester (50 mM)	8	0.25	Very basic	0.40
Butyramide (100 mM)	7	130		0
Butyric acid (100 mM)	6	8.7		0
Methyl butyrate (50 mM)	7	0.20	Very basic	0.10
Nicotinamide (100 mM)	7	9.9		0
Nicotinic acid (25 mM)		0		0
dl-Lactamide (100 mM)	7	121	7	0.03
dl-Lactic acid (100 mM)	6	0.17	Very basic	0.60

^aHydroxylamine (500 mM) was used as the acyl acceptor.