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. 2023 Nov 26;13:20770. doi: 10.1038/s41598-023-48073-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Isolation and characterization of CAFs. (a) The outgrowth of fibroblastic cells from tumor tissue began on day two and reached 80% confluence on day 21. These cells were passaged and maintained for Curcumin treatment and co-culture experiments. (b) Passage 2 or 3 CAFs were used in all experiments. Fibroblastic colonies were cultured in 4-well plates and immune stained with rabbit anti-human FAP, and (c) nuclei were counterstained with DAPI. Cells showed expression of FAP.