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Purification of cloned NagA
| Fraction | Total protein (mg) | Total activity (U) | Sp act (U/mg of protein) | Purification factor | Yield (%) |
|---|---|---|---|---|---|
| Crude extract | 53.6 | 412.3 | 7.2 | 1.0 | 100.0 |
| DEAE-Toyopearl 650M | 9.6 | 384.1 | 40.2 | 5.6 | 93.2 |
| Sephadex G-100 | 6.7 | 357.6 | 53.1 | 7.4 | 86.7 |
| Bio-scale Q2 | 4.4 | 283.3 | 64.4 | 8.7 | 68.7 |
