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Substrate specificity of NagA
| Substrate | Relative rate (%)a |
|---|---|
| PNP-β-GlcNAc | 100.0 |
| PNP-α-GlcNAc | 0.0 |
| PNP-β-GalNAc | 2.6 |
| PNP-β-Gal | 0.0 |
| PNP-β-Glu | 0.0 |
| PNP-β-Xyl | 0.0 |
| PNP-β-Cel | 0.0 |
| Di-N-acetylchitobiose | 13.0 |
| Tri-N-acetylchitotriose | 72.0 |
| Tetra-N-acetylchitotetraose | 69.0 |
| Penta-N-acetylchitopentaose | 100.0 |
| Hexa-N-acetylchitohexaose | 31.0 |
Rate relative to activity with PNP-β-GlcNAc or penta-N-acetylchitopentaose as a substrate, which was taken as 100%. The enzyme activity was 12 mU.
