Skip to main content
. 2023 Nov 13;11:1297910. doi: 10.3389/fcell.2023.1297910

FIGURE 7.

FIGURE 7

Activation of canonical Wnt or TGF-β/BMP signaling pathways enhances larval melanocyte regeneration (A) Scheme for experimental design of melanocyte regeneration. Zebrafish embryos were treated with 4-HA from 36 hpf to 60 hpf. After washout of 4-HA at 60 hpf, drugs were administered into the embryo water, and larvae were analyzed at 4 dpf. (B) 2 dpf zebrafish larvae treated with DMSO or 4-HA. (C) Dot plot showing the number of melanocytes in 4 dpf larvae treated with DMSO, 4-HA, 4-HA + BIO, or 4-HA + ISL. Each dot represents one larva (DMSO n = 16, 4-HA n = 19, 4-HA + BIO n = 18, 4-HA + ISL n = 17). Statistical significance was evaluated using a one-way ANOVA test **p < 0.01 and ****p < 0.0001. (D) Representative images of melanocyte regeneration groups are counted in (C). 4-HA, 4-Hydroxyanisole; DMSO, dimethyl sulfoxide; hpf, hours post-fertilization; dpf, days post-fertilization; ISL, isoliquiritigenin.