Figure 11.

L-NAME reduces nitric oxide levels in aged BFCNs. Rat BFCNs were treated with either 1 mM L-NAME or vehicle for 24 h prior to staining with 5 μM DAF-FM to detect nitric oxide levels in the cell bodies. (A–C) Aged (DIV19) BFCNs treated with 1 mM L-NAME for 24 h (C) exhibited reduced DAF-FM fluorescence (green) compared to aged BFCNs treated with vehicle (B). L-NAME treatment restored DAF-FM fluorescence to the levels seen in young (DIV7) vehicle-treated BFCNs (A). DAPI is shown in blue. (D) Quantification of the images shown in A–C, one-way ANOVA and post hoc Tukey test. N = 10 images/group. For each group, images were obtained from one chamber. No outliers were identified using ROUT (Q = 1%). Error bars represent SEM. **p < 0.01, ****p < 0.0001. DIV, days in vitro. Scale bars: 10 μm.