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. 2023 Nov 13;16:1241420. doi: 10.3389/fnmol.2023.1241420

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Copyright © 2023 Kropf, Shekari, Jaberi, Puri, Wu and Fahnestock.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rat and mouse BFCNs aged in vitro stain positive for senescence-associated beta-galactosidase. Aged (DIV17-21) and young (DIV7-10) basal forebrain neurons were stained for senescence-associated beta-galactosidase, a well-validated marker of cellular aging, using a colorimetric staining kit. (A) Senescence-associated beta-galactosidase is undetectable in young rat BFCNs. (B) Rat basal forebrain neurons aged in vitro stain positive for senescence-associated beta-galactosidase (red). DIV: days in vitro. Scale bars: 10 μm. (C) Senescence-associated beta-galactosidase is undetectable in young mouse BFCNs. (D) Mouse basal forebrain neurons aged in vitro stain positive for senescence-associated beta-galactosidase (purple). DIV, days in vitro. Scale bars: 10 μm.