TABLE 2.
Dehalogenase activities in crude extracts prepared from cells grown on different carbon sourcesa
| Enzyme substrate | Dehalogenase sp act (mU/mg of protein) with the following carbon sources:
|
|||
|---|---|---|---|---|
| Citrate | 1-Chloro- butane | cis-3-Chloro- acrylic acid | trans-3-Chloro- acrylic acid | |
| cis-1,3-Dichloropropene | 440 | 280 | 280 | 256 |
| trans-1,3-Dichloropropene | 238 | 203 | 167 | 147 |
| cis-3-Chloroallyl alcohol | <10 | <10 | <10 | <10 |
| trans-3-Chloroallyl alcohol | <10 | <10 | <10 | <10 |
| cis-3-Chloroacrylic acid | <10 | <10 | 161 | <10 |
| trans-3-Chloroacrylic acid | 230 | 63 | 134 | 177 |
a
Specific activities with various substrates (5 mM) were determined with extracts prepared from cells grown on different carbon sources.