TABLE 4.
Dehalogenating activities of crude extracts and purified dehalogenase
| Enzyme substrate | Dehalogenase sp act (mU/mg of protein)a
|
||
|---|---|---|---|
| Cell extract of strain 170 | Cell extract of BL21(DE3)/ pGEF(dhaA) | Purified de- halogenase | |
| cis-1,3-Dichloropropene | 440 | 4,204 | 7,150 |
| trans-1,3-Dichloropropene | 238 | 2,140 | 3,549 |
| 1-Chloropropane | 48 | 449 | 744 |
| 1-Chlorobutane | 79 | 711 | 1,232 |
| cis-3-Chloroacrylic acid | NDb | <10 | <10 |
| trans-3-Chloroacrylic acid | 230 | <10 | <10 |
a
Halide production at 30°C with different substrates (5 mM) was determined at pH 8.2.
b
ND, not determined, because the enzyme is not expressed under the growth conditions used.