TABLE 4.
Enzyme substrate | Dehalogenase sp act (mU/mg of protein)a
|
||
---|---|---|---|
Cell extract of strain 170 | Cell extract of BL21(DE3)/ pGEF(dhaA) | Purified de- halogenase | |
cis-1,3-Dichloropropene | 440 | 4,204 | 7,150 |
trans-1,3-Dichloropropene | 238 | 2,140 | 3,549 |
1-Chloropropane | 48 | 449 | 744 |
1-Chlorobutane | 79 | 711 | 1,232 |
cis-3-Chloroacrylic acid | NDb | <10 | <10 |
trans-3-Chloroacrylic acid | 230 | <10 | <10 |
Halide production at 30°C with different substrates (5 mM) was determined at pH 8.2.
ND, not determined, because the enzyme is not expressed under the growth conditions used.