Skip to main content
. 2020 Apr 12;147(8):dev184655. doi: 10.1242/dev.184655

Fig. 6.

Fig. 6. EWSR1 is a protein-binding partner of TRIM71 in mouse testes. (A) Silver staining of proteins isolated from TRIM71 IP of P10 testis lysate and identified by MS. (B) Enrichment of TRIM71 in EWSR1 and CSNK2A2 IP using P10 mouse testes. (C,D) Co-immunofluorescence of TRIM71 and EWSR1 (C) or CSNK2A2 (D) in cryostat sections from P10 and adult testes. (E-H) Western blot (E) and quantification of relative expression levels of TRIM71 (F), EWSR1 (G) and CSNK2A2 (H) after TRIM71 knockdown by shRNA treatment. Data in F-H are mean±s.e.m. for three different primary cultures and two separate knockdowns. *P<0.05, **P<0.01 (Student's t-test). Scale bars: 20 μm.

EWSR1 is a protein-binding partner of TRIM71 in mouse testes. (A) Silver staining of proteins isolated from TRIM71 IP of P10 testis lysate and identified by MS. (B) Enrichment of TRIM71 in EWSR1 and CSNK2A2 IP using P10 mouse testes. (C,D) Co-immunofluorescence of TRIM71 and EWSR1 (C) or CSNK2A2 (D) in cryostat sections from P10 and adult testes. (E-H) Western blot (E) and quantification of relative expression levels of TRIM71 (F), EWSR1 (G) and CSNK2A2 (H) after TRIM71 knockdown by shRNA treatment. Data in F-H are mean±s.e.m. for three different primary cultures and two separate knockdowns. *P<0.05, **P<0.01 (Student's t-test). Scale bars: 20 μm.