Skip to main content
. 2023 Oct 5;18(11):2123–2137. doi: 10.1016/j.stemcr.2023.09.002

Figure 2.

Figure 2

Effect of input cell compostion and cell culture media on EHT properties

(A and B) Pearson correlation of (A) force and (B) resting length of EHTs with percentage of cTNT-positive input cells for EHT generation. OCTN2 (+/+), n = 10; OCTN2 (N32S), n = 7; OCTN2 (−/−), n = 7 differentiation batches. Each replicate represents the mean value of 7–20 EHTs for the specific differentiation batch.

(C) EHT force development in fatty acid medium. Serum-free cell culture medium was supplemented with 50 μM carnitine, linoleic acid- and oleic acid-albumin. Data are normalized to baseline force. OCNT2 (+/+), n = 11 EHTs from 2 batches; OCTN2 (N32S), n = 11 EHTs from 2 batches; OCTN2 (−/−), n = 12 EHTs from 2 batches. Two-way ANOVA vs. OCNT2 (+/+) followed by Bonferroni’s post test for multiple comparisons, ∗p < 0.05. Data are expressed as mean ± SEM.

(D) Difference in ΔGlucose medium concentration divided by product of individual spontaneous beating frequency × force. ΔGlucose = glucose concentration at baseline minus glucose concentration after 24 h of incubation in medium containing 5.5 mM glucose and 10% horse serum. OCNT2 (+/+), n = 59 EHTs from 5 batches; OCTN2 (N32S), n = 51 EHTs from 4 batches; OCTN2 (−/−), n = 28 EHTs from 4 batches. One-way ANOVA followed by Bonferroni’s post test for multiple comparisons, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. One data point represents one EHT. Data are expressed as mean ± SEM. See also Figure S3.