Figure 5.

NKX2.5 binds a majority of ISL1 peaks and cooperates with ISL1 to regulate gene expression in CPs

(A) Venn diagram of NKX2.5 and ISL1 ChIP-seq peaks in WT day 6 CPs.

(B) Example ChIP-seq tracks showing loci bound by ISL1, NKX2.5, or both in day 6 CPs. Putative NPPA/NPPB enhancer labeled as related to Figure S5I. Data shown from single representative replicate.

(C) Hierarchical UMAP scRNA-seq clustering of WT, ISL1^−/−, and NKX2.5^−/− day 8 CPs (n = 3 independent experiments).

(D) Expression of MYL9 in day 8 CPs, superimposed on the UMAP from (C).

(E) FeaturePlot display of the distribution of WT and ISL1^−/− day 8 CPs across the clusters depicted in (C).

(F) Heatmap of significantly dysregulated genes with biological GO terms in day 8 ISL1^−/− or NKX2.5^−/− CPs compared with WT.