Figure 2.

UGT1A1 is negatively regulated by glucocorticoid signaling

(A) Bright-field images of organoids treated with 10 mg/L bilirubin and agonists: hydrocortisone (1 μM) and dexamethasone (1 μM). Scale bars, 200 μm.

(B) Bilirubin assay measuring unconjugated and conjugated bilirubin in the samples in (A). n = 9 independent experiments.

(C) Bright-field images of organoids treated with 10 mg/L bilirubin and antagonists: ketoconazole (1 μM) and mifepristone (1 μM). Scale bars, 200 μm.

(D) Bilirubin assay measuring unconjugated and conjugated bilirubin in the samples in (C). n = 9 independent experiments.

(E) Schematic for XRE element upstream of UGT1A1 gene depicting how GCR and MECP2 affect expression of UGT1A1.

(F) NR3C1-MECP2 ChIP-reChIP-PCR for organoids treated with 10 mg/L bilirubin and mifepristone (1 μM) or dexamethasone (1 μM).

(G) NR3C1-MECP2 ChIP-reChIP-qPCR for samples in (F). Data are mean ± SD, n = 9 independent experiments.

ns, p > 0.05, ^∗p ≤ 0.05, ^∗∗p ≤ 0.01, ^∗∗∗p ≤ 0.001, and ^∗∗∗∗p ≤ 0.0001.