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. 2023 Oct 19;18(11):2222–2239. doi: 10.1016/j.stemcr.2023.09.011

Figure 2.

Figure 2

GluA2-lacking AMPA receptors are expressed in an early stage of homeostatic plasticity

(A) Schematic representation of tetrodotoxin (TTX) treatment and 1-naphthyl acetyl spermine trihydrochloride (NASPM) treatment workflow.

(B) Representative raster plots showing 1 min of spontaneous activity from Ctrl2 neuronal networks grown on microelectrode arrays (MEAs). Where indicated, the neurons were vehicle-treated (Veh), or neurons were first treated with 1 μM TTX for 12 h and then TTX was removed (TTX-12 h), or neurons were treated with 10 μM NASPM after TTX was removed (TTX-12 h+NASPM).

(C) Bar graphs showing the quantification of mean network burst rate (NBR) and mean burst rate (BR) for (B). n = number of MEA wells/batches: Veh n = 4/1, TTX-12 h n = 4/1, TTX-12 h+NASPM n = 4/1.

(D) Representative raster plots showing 1 min of spontaneous activity from Ctrl1 neuronal networks grown on MEAs. Where indicated, the neurons were vehicle-treated (Veh), or neurons were first treated with 1 μM TTX for 24 h and then TTX was removed (TTX-24 h), or neurons were treated with 10 μM NASPM after TTX was removed (TTX-24 h+NASPM).

(E) Bar graphs showing the quantification of NBR and BR for (D). n = number of MEA wells/batches: Veh n = 4/1, TTX-24 h n = 4/1, TTX-24 h+NASPM n = 4/1.

(F) Representative raster plots showing 1 min of spontaneous activity from Ctrl1 neuronal networks grown on MEAs. Where indicated, the neurons were vehicle-treated (Veh), or neurons were first treated with 1 μM TTX for 48 h and then TTX was removed (TTX-48 h), or neurons were treated with 10 μM NASPM after TTX was removed (TTX-48 h+NASPM).

(G) Bar graphs showing the quantification of NBR and BR for (F). n = number of MEA wells/batches: Veh n = 4/1, TTX-48 h n = 4/1, TTX-48 h+NASPM n = 4/1. Data represent means ± SEM. ∗p < 0.05, ∗∗p < 0.005, ∗∗∗p < 0.0005, one-way ANOVA test followed by a post hoc Bonferroni correction was performed between conditions. All means, SEMs, and test statistics are listed in Table S3.